Abstract: 2205 stainless steel as a second generation stainless steel was widely used in industrial fields such as oil pipelines and offshore platforms with high corrosion resistance. Directional solidification can reveal the solidification process of stainless steel and provides a basis for studying solute segregation during solidification. The types of micro-zone analysis methods can be divided into two categories: wave spectroscopy and energy spectroscopy. Solute segregation at the solid-liquid interface of 2205 stainless steel material was analysed and compared with electronic probe（EPMA） equipped with wave spectrometer（WDS） and energy spectrometer（EDS） respectively to investigate the differences in the accuracy of microscopic solute segregation results between different instruments. The results show that the electronic probe equipped with a wave spectrometer can reflect the elemental change pattern at the solid-liquid interface more intuitively than the energy spectrum,and its determination results have higher accuracy.